MOPS BUFFER- Need ADVICE

Mano mdep at musica.mcgill.ca
Sat Nov 13 23:30:52 EST 1999


Hi all,
    If the MOPS buffer for running formaldehyde gels to separate RNA is
"bad", will it affect integrity of RNA?  Or else, does it affect the
migration of RNA? How about pH?
I have apparent degradation on my gel, but I beleive my buffer is bad..
is this possible? or is it just smearing?  I'll repeat it with new
buffer anyways.

Thanks.






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