DNA isolation from BCG
drag at eskulap.am.lublin.pl
Sun Nov 14 10:50:27 EST 1999
Few weeks ago I started PCR test detecion of Mycobacterium
tuberculosis in tuberculosis patients,
but in electrophoresis I got only bands of positiv control.
I have investigate about 20 samples obtained from patients with
suspipitons of pulmonary and extra-pulmonary tuberculosis
(sputum, pleural effusion, bronchaial washing).
I suspect there must be sth wrong with isolation protocol or
concentration of BCG.
I wil be grateful for any help.
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