DNA isolation from BCG

Ma Sheila M. De Jesus mshmdeje at USTCC.UST.EDU.PH
Mon Nov 15 03:05:30 EST 1999

I used the Genereleaser protocol for isolation of mycobacterial DNA and it
worked perfectly.

On Sun, 14 Nov 1999, Mick wrote:

> Few weeks ago I started PCR test detecion of  Mycobacterium
> tuberculosis  in tuberculosis patients,
> but in electrophoresis I got only bands of positiv control.
> I  have investigate about 20 samples obtained from patients with
>  suspipitons of pulmonary and extra-pulmonary tuberculosis 
> (sputum, pleural effusion, bronchaial washing).
> I suspect there must be sth wrong with isolation protocol or
> concentration of BCG.
> I wil be grateful for any help.
>  Michal Fik

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