DNA isolation from BCG
Ma Sheila M. De Jesus
mshmdeje at USTCC.UST.EDU.PH
Mon Nov 15 03:05:30 EST 1999
I used the Genereleaser protocol for isolation of mycobacterial DNA and it
On Sun, 14 Nov 1999, Mick wrote:
> Few weeks ago I started PCR test detecion of Mycobacterium
> tuberculosis in tuberculosis patients,
> but in electrophoresis I got only bands of positiv control.
> I have investigate about 20 samples obtained from patients with
> suspipitons of pulmonary and extra-pulmonary tuberculosis
> (sputum, pleural effusion, bronchaial washing).
> I suspect there must be sth wrong with isolation protocol or
> concentration of BCG.
> I wil be grateful for any help.
> Michal Fik
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