T-tail

Daniel.Marc marc at tours.inra.fr
Mon Nov 15 04:07:56 EST 1999


Jrchukarmo Schrader (Jarmo.Schrader.removethis at genfys.slu.se) wrote:
: In article <382A2582.1E68E202 at stromix.com>, sean at stromix.com wrote:

: > Can someone give me a protocol for adding a 3' T overhang to a cut
: > vector for TA cloning of PCR products.


Marchuk etal. (1990) Construction of T-)vectors, a rapid and 
general system for direct cloning of unmodified PCR-products.
Nucl. Acids Res. vol 19, p1154.





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