Polyethyleneimine precipitation protocol

Wolfgang Schechinger Wolfgang.Schechinger at med.uni-tuebingen.de
Mon Nov 15 05:58:44 EST 1999


Eugene, 

Just a silly thouhght:
Why not destroying DNA with DNAse and then inactivating DNAse by a
heat step (that Taq should tolerate). (It might be more favoravable to
add DNAse NOT in the last purification step but after the first crude
enrichment). Where are the implications?

Wolfgang

> From:          Kulikov Eugene <eumenius at imb.ac.ru>
> Subject:       Polyethyleneimine precipitation protocol
> Date:          Sun, 14 Nov 1999 23:30:57 +0300
> Organization:  MTU-Intel ISP
> To:            methods at hgmp.mrc.ac.uk

> Hello all,
> 
> I recently began to produce recombinant Taq-polymerase and the first
> problem I have reached is purification of enzyme of DNA traces. Does
> somebody know the protocol of DNA precipitation with
> polyethyleneimine (Polymine P)?
> 
> Best regards - Kulikov Eugene
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usual disclaimers apply 
-----                              
Dr. Wolfgang Schechinger   
Pathobiochemistry Dept.      
University of Tuebingen, Germany
email: wgschech at med.uni-tuebingen.de 
wwWait: http://www.medizin.uni-tuebingen.de/~wgschech/start.htm
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*unsolicited mail is *NOT* appreciated
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