Effects of Uridine on Restriction Enzyme

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Mon Nov 15 05:00:29 EST 1999

In article <382c7f4f$0$97654 at news.execpc.com>, Scott C. Johnson
<scjohns at execpc.com> writes
>Anyone experienced with the effects of uridine incorporation on
>restriction enzyme recognition and cutting? If you could point me to
>some references or would like to discuss it please drop me a line.

I'm sure there was a paper in NAR or Gene about this some time ago. Try
PubMed. Alternatively PCR one of the super polylinkers (all 64 hexameric
palindromic RE sites) using Taq and dUTP and universal + reverse primer
and see what cuts etc.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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