chimeric cDNA clones : HELP

dgdsg bobby at ucla.edu
Mon Nov 15 21:05:31 EST 1999


Hi.

I clone 16S rRNA genes and it gives a lot of chimeric clones :

Isolation of RNA with a RNAwiz kit from Ambion, DNase treatment, RT-PCR, PCR 
and clone that. 

Any tricks out there??????

Thanks.

Jacob Hofman




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