Difficulties in expression of protein in E. coli

sudhanshu vrati vrati at nii.res.in
Tue Nov 16 01:47:21 EST 1999


We are using a T7 promoter based expression vector in BL21 cells for
expressing a viral protein.  The protein was successfully expressed,
purified and sequenced to find it was the correct one.  But now the same
vector in same cells refuses to make the desired protein.  Any clues ?


Sudhanshu






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