Hallo, Does anybody know what is the minimum number of nucleotides before the AUG in a mRNA that in vitro translation in an eukaryotic system is still working ? Thanks in advance Joerg -- Joerg A.Schenk Max-Delbrueck-Centrum fuer Molekulare Medizin (MDC) Robert-Roessle-Strasse 10 D-13125 Berlin-Buch, GERMANY Tel.: +49 (30) 9406 2169/2662 Fax.: +49 (30) 9406 3895