In Vitro Translation

Dr. David J. Meyer meyerdj at
Tue Nov 16 15:01:22 EST 1999

Joerg Schenk <jschenk at> wrote:


>Does anybody know what is the minimum number of nucleotides before the
>AUG in a mRNA that in vitro translation in an eukaryotic system is still
>working ?

>Thanks in advance

>Joerg A.Schenk
>Max-Delbrueck-Centrum fuer Molekulare Medizin (MDC)
>Robert-Roessle-Strasse 10
>D-13125 Berlin-Buch, GERMANY
>Tel.: +49 (30) 9406 2169/2662
>Fax.: +49 (30) 9406 3895

I know that 28 nt is sufficient in vivo in yeast.

David J. Meyer, Ph.D.
Quality Traits
Pioneer Hi-Bred International, Inc.
7300 NW 62nd Ave.
Johnston, IA   50131-1004

Ph. 515/254-2639
FAX 515/254-2619
Email: meyerdj at

More information about the Methods mailing list