Maxam-Gilbert Who?!

CSC un691cs at genius.embnet.dkfz-heidelberg.de
Wed Nov 17 03:33:18 EST 1999


On Tue, 16 Nov 1999, Jens Tornoe wrote:

> How come you do not use Sanger sequencing? It is a lot easier to do, and you
> ought to be able to design a sequencing primer that would give the same
> result as a Maxam-Gilbert reaction.
 
> M-G is obsolete, as far as I know. Guess that is why you can't find it
> anywhere. If other people out there find me to be wrong, I would appreciate
> your correction.

MG sequencing is useful for RNAs for instance. Sanger will not always work
if you have strong secundary structures. (For this reason we will probably
only get 99% of the human genome sequenced, hihihi.)

Kind regards, 

Clemens Suter-Crazzolara, PhD
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