making protoplasts from gram positive cells
kaerst at gbf.de
Wed Nov 17 04:31:51 EST 1999
we tried to make protoplasts of listeria using egg white lysozyme - that
worked, but took at least 16 hours which is much too long - one or two
hours are required.
After some serching I found that Sigma offers lysostaphin, a muramidase
from Staphylococcus - that might help, but it is very expensive. Is there
any experience with this enzyme? Or are others available for this purpose
with much better performance than egg white lysozyme?
Thanks a lot
Uwe Kaerst, Ph.D.
Dept. Cell Biology
GBF - Gesellschaft fuer Biotechnologische Forschung
Mascheroder Weg 1
D-38124 Braunschweig, Germany
Tel: +(49) 531 6181318
Fax: +(49) 531 2612313
EMAIL: kaerst at gbf.de
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