making protoplasts from gram positive cells

Uwe Kaerst kaerst at gbf.de
Wed Nov 17 04:31:51 EST 1999


Hi,

we tried to make protoplasts of listeria using egg white lysozyme - that 
worked, but took at least 16 hours which is much too long - one or two 
hours are required.

After some serching I found that Sigma offers lysostaphin, a muramidase 
from Staphylococcus - that might help, but it is very expensive. Is there 
any experience with this enzyme? Or are others available for this purpose 
with much better performance than egg white lysozyme?

Thanks a lot

Uwe



-- 
Uwe Kaerst, Ph.D.
Dept. Cell Biology
GBF  - Gesellschaft fuer Biotechnologische Forschung
Mascheroder Weg 1
D-38124 Braunschweig, Germany

Tel:  +(49) 531 6181318
Fax: +(49) 531 2612313

EMAIL: kaerst at gbf.de
Company:     http://www.gbf.de/Welcome.html




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