Immunoprecip gurus?

Stephen Snowdy stephen_snowdy at med.unc.edu
Wed Nov 17 15:28:03 EST 1999


Ok, let's say protein A binds to protein B which binds to protein C.  If
I pull down with an antibody to protein C (from cell lysates), should I
expect to be able to pull down protein A with it and probe for protein A
on a Western, or is it too much to hope for that everthing will stay
together during the IP?  I'm sure it depends on dissociation contants,
but generally speaking...

Are magnetic beads more effiecient than Protein A Sepharose beads?
-- 
Stephen Snowdy
Department of Pharmacology
College of Medicine
University of North Carolina-Chapel Hill
(919) 966-0905 Voice
(919) 966-0907 Fax




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