MOPS BUFFER- Need ADVICE

Mano mdep at musica.mcgill.ca
Thu Nov 18 00:25:05 EST 1999


THANKS all, I corrected the pH and the RNA separated nicely.... thanks for the
discussion!!!!!

"Bernard Murray, PhD" wrote:

> In article <38317392.A5C5D0C2 at uni-konstanz.de>,
> Frank.Fackelmayer at uni-konstanz.de wrote:
>
> > "Bernard Murray, PhD" wrote:
> >
> > > In article <3830525A.A98B327C at unix.ccc.nottingham.ac.uk>, james stevenson
> > > <pcxjs at unix.ccc.nottingham.ac.uk> wrote:
> > >
> > > > If its gone yellow, then its decomposed.
> > >
> > > This is nonsense.  Clear straw-yellow MOPS solutions are just fine
> > > (I consider it a helpful indication that it has been autoclaved
> > > - although MOPS buffer will turn yellow spontaneously on exposure
> > > to air).
> >
> > I don´t think it is nonsense to say that the yellow color of MOPS results from
> > its decomposition.
>
> Sorry, the original context was that the "decomposed" buffer was unusable
> and that was the point I was disputing.  I agree that something must have
> changed if the colour is different.  It still acts as a buffer and has
> no detrimental effect upon RNA.
>
> > That said, I have to admit that I once used autoclaved MOPS after 2 years on
> > the shelf at RT (dark yellow!), and had no problems at all. Apparently
> > decomposition only affects a small proportion of the stuff and there is enough
> > intact MOPS left....
>
> That was exactly my point.  You can buy commercial MOPS solutions
> and all the suppliers I have seen autoclave their offerings so
> the solutions are yellow.  I've used solutions several years old
> without any problems (stored room temperature out of direct sunlight).
>
>    Bernard
>
> --
> Bernard P. Murray, PhD
> bpmurray at cgl . ucsf . edu
> Department of Cellular & Molecular Pharmacology, UCSF





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