Half life of TdT

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Thu Nov 18 04:21:32 EST 1999

In article <l03130307b45a38c6293c@[]>, alex dobrovic
<adobrovic at medicine.adelaide.edu.au> writes
>Does anyone know what the half life of terminal deoxy transferase is? Can
>you use 20U for 30 minutes instead 40U for 15 minutes? Or are longer tails
>added to fewer substrates?
>adobrovi at medicine.adelaide.edu.au

You can tail for 90mins if you wish. A lot of RACE protocols use that
long. For more info. On length of tails have a look at Methods in
Enzymology v 100 pp96-116. 

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

More information about the Methods mailing list