Half life of TdT

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Thu Nov 18 04:21:32 EST 1999


In article <l03130307b45a38c6293c@[129.127.90.187]>, alex dobrovic
<adobrovic at medicine.adelaide.edu.au> writes
>Does anyone know what the half life of terminal deoxy transferase is? Can
>you use 20U for 30 minutes instead 40U for 15 minutes? Or are longer tails
>added to fewer substrates?
>
>Regards
>
>Alex
>
>
>adobrovi at medicine.adelaide.edu.au
>
>

You can tail for 90mins if you wish. A lot of RACE protocols use that
long. For more info. On length of tails have a look at Methods in
Enzymology v 100 pp96-116. 

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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