Coomassie staining of proteins on PVDF

[Thorsten Burmester]

I asked this question in bionet.molbio.proteins but it may be better
here:

After SDS-PAGE and Western transfer to PVDF I stained the proteins with
0.1% Coomassie Blue in H20 dd. Destaining with 50% methanol. To my
surprise the band of interest was _not_ stained but remained white on a
light blue background. Other proteins were stained well as expected. Any
explanation?  

TIA,

Thorsten

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Thorsten Burmester
thorsten at erfurt.thur.de