Coomassie staining of proteins on PVDF

Thorsten Burmester thorsten at erfurt.thur.de
Thu Nov 18 04:47:59 EST 1999


I asked this question in bionet.molbio.proteins but it may be better
here:

After SDS-PAGE and Western transfer to PVDF I stained the proteins with
0.1% Coomassie Blue in H20 dd. Destaining with 50% methanol. To my
surprise the band of interest was _not_ stained but remained white on a
light blue background. Other proteins were stained well as expected. Any
explanation?  

TIA,

Thorsten

--
Thorsten Burmester
thorsten at erfurt.thur.de




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