Coomassie staining of proteins on PVDF

Thorsten Burmester thorsten at erfurt.thur.de
Thu Nov 18 13:50:36 EST 1999


Tyson wrote:
> 
> ....how long did you stain/destain the gel?
                                         ^^^ the membrane

1' 0.1 % Coomassie, 30' 50% methanol.   

> ....perhaps there is too litlle of the desired protein present to pick up
> the stain or the destaining is too harsh

No. Normal staining of the gel. The band on PVDF is "negatively"
stained.

Thorsten

--
Thorsten Burmester
thorsten at erfurt.thur.de




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