Coomassie staining of proteins on PVDF

[Thorsten Burmester]

Tyson wrote:
> 
> ....how long did you stain/destain the gel?
                                         ^^^ the membrane

1' 0.1 % Coomassie, 30' 50% methanol.   

> ....perhaps there is too litlle of the desired protein present to pick up
> the stain or the destaining is too harsh

No. Normal staining of the gel. The band on PVDF is "negatively"
stained.

Thorsten

--
Thorsten Burmester
thorsten at erfurt.thur.de