sjin at LELAND.STANFORD.EDU
Thu Nov 18 21:33:46 EST 1999
Yes, You definitely need to repeat the differential display at least
for two or three times. Since you will need to do northern to further
confirmed if those differential displayed bands are represent the
differential expressed mRNAs. It is saving you time for the long run
to make sure those differentially displayed bands are reproducible.
When you do that, you have to make sure every time you are using
exactly same condition (even the same PCR machine and same Taq
enzyme) I had experience that I got different band patterns after I
changed to a different brand Taq. This technique is very very
sensitive and it is sometimes hard to get a very reproducible result.
But, if you are careful enough, it should work.
>I found several differentially expressed bands. Should I try and repeat
>this result before attempting to clone/sequence? I'd appreciate it if
>someone could give me
Department of Anesthesia
Palo Alto, CA 94305
sjin at stanford.edu
More information about the Methods