HELP: Yeast 3-hybrid problem
mayr at SALK.EDU
Thu Nov 18 23:34:18 EST 1999
I'm trying to do a yeast 3-hybrid screen using Clontech's pBridge and
pACT2 vectors. My problem is, that whatever vectors I transform the
yeast always grows on -His media, even when I transform empty pBridge
and pACT2 vectors and rise 3-AT to > 200mM. The yeast wt-phenotype is
OK (tried CG1945 and Y190), I repeated my transformation with a
complete new set of reagents and media, still the same result.
Has anybody got an idea what's wrong ?
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