HELP: Yeast 3-hybrid problem

[Bernhard Mayr]

Hi,

I'm trying to do a yeast 3-hybrid screen using Clontech's pBridge and 
pACT2 vectors. My problem is, that whatever vectors I transform the 
yeast always grows on -His media, even when I transform empty pBridge 
and pACT2 vectors and rise 3-AT to > 200mM. The yeast wt-phenotype is 
OK (tried CG1945 and Y190), I repeated my transformation with a 
complete new set of reagents and media, still the same result.
Has anybody got an idea what's wrong ?


Bernhard Mayr
The Salk Institute
Peptide Biology Labs
10010 North Torrey Pine Road
La Jolla, CA 92037
USA

Fon +1 858 453 4100 ext 1176