Hydroxyapatite, methods and sources???

Narendra Kaushik n.kaushik at ic.ac.uk
Fri Nov 19 08:30:38 EST 1999


It was the method of choice before PCR  to make subtracted libraries.
Any how you can find it in: Gene Transfer and Expression - A lab manual
by Kriegler, WH Freeman and Co.1990 ISBN 0-7167-7004-0. You can buy
hydroxyapatite and column are from BIO-RAD, there must be another
suppliers too. There are  couple of publications by Brento Soares,
creator of IMAGE libraries.
Bonaldo MF, et al.  Normalization and subtraction: two approaches to
facilitate gene discovery. Genome Res. 1996. 6, 791-806.

Sagerstrom CG et al. Subtractive Cloning: Past, Present and Future. Ann
Rev. Biochem,
1997. 66: 751-783. A good rev on this matter
There is also paper in PNAS by Soares about normalization using HAP
chromatography.

Narendra Kaushik
Imperial College at Charing Cross Campus,
St Dunstan's Road,
London W6 8RP
UK





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