Crosslinking with NaBorohydride

[Miguel Zamora]

I understand that there is a way of "freezing" a Shiff's base formed in
a enzyme-substrate intermediate complex by using Sodium Borohydride as a
crosslinker.  If I'm not mistaken the crosslinker breakes the -N=C-  to
form a -N-C- which is very stable and irreversible.  Does anybody have
any experience with this?  I'm trying to identify the enzyme that
catalyzes my substrate and this could be a good system.  Thanks.

Miguel
-------------- next part --------------
A non-text attachment was scrubbed...
Name: mzamora.vcf
Type: text/x-vcard
Size: 291 bytes
Desc: Card for Miguel Zamora
Url : http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/19991119/2a19acfc/mzamora.bin