Crosslinking with NaBorohydride

Miguel Zamora mzamora at bcm.tmc.edu
Fri Nov 19 15:38:33 EST 1999


I understand that there is a way of "freezing" a Shiff's base formed in
a enzyme-substrate intermediate complex by using Sodium Borohydride as a
crosslinker.  If I'm not mistaken the crosslinker breakes the -N=C-  to
form a -N-C- which is very stable and irreversible.  Does anybody have
any experience with this?  I'm trying to identify the enzyme that
catalyzes my substrate and this could be a good system.  Thanks.

Miguel
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