yoel and adaia shiboleth
shibolet at mishkei.org.il
Fri Nov 19 17:39:12 EST 1999
This is the most commonplace problem with Northerns. Bring down the salt or
bring the temp. up on your washes (or both). By the way, I have even seen
the unexplained phenomena that the 28/18S bands act differentially as would
be expected only by the proper size band...
> HI all,
> Problem, 18 and 28 S rRNA severly cross react with my probe on
> northern (bad news), and I get no backrground reactivity with the
> membrane (which is the only good news). I do not beleive that it is due
> to my probe being non-specific. What are the most liekly problems?
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