Drug-induced necrosis

[Menoret]

Why don't you treat your cells with something really tough, like
distilled water ? This is a well-known protocol, the cells explose and I
bet that you won't see apoptosis.

Antoine Menoret


"Ian A. York" wrote:
> 
> I thought this was going to be an easy one to find in medline, but boy was
> I wrong.
> 
> I want to induce necrotic cell death in several lines of cultured cells.
> I want this to be all necrosis, all the time; no apoptosis sneaking in
> here.  And I want it to be a well-known protocol, so that we don't have to
> spend a lot of time justifying the system and running all the various
> controls to prove it's pure necrosis.
> 
> But when I try to do searches, all I can find is people saying things
> like, "unlike necrotic cell death, Drug X induced apoptosis ... "
> 
> I guess no one cares about boring old necrosis, but surely someone must
> have done a control some time.
> 
> Any tips?
> 
> Thanks.
> 
> Ian
> --
>     Ian York   (iayork at panix.com)  <http://www.panix.com/~iayork/>
>     "-but as he was a York, I am rather inclined to suppose him a
>      very respectable Man." -Jane Austen, The History of England