menoret at home.com
Sat Nov 20 19:32:13 EST 1999
Why don't you treat your cells with something really tough, like
distilled water ? This is a well-known protocol, the cells explose and I
bet that you won't see apoptosis.
"Ian A. York" wrote:
> I thought this was going to be an easy one to find in medline, but boy was
> I wrong.
> I want to induce necrotic cell death in several lines of cultured cells.
> I want this to be all necrosis, all the time; no apoptosis sneaking in
> here. And I want it to be a well-known protocol, so that we don't have to
> spend a lot of time justifying the system and running all the various
> controls to prove it's pure necrosis.
> But when I try to do searches, all I can find is people saying things
> like, "unlike necrotic cell death, Drug X induced apoptosis ... "
> I guess no one cares about boring old necrosis, but surely someone must
> have done a control some time.
> Any tips?
> Ian York (iayork at panix.com) <http://www.panix.com/~iayork/>
> "-but as he was a York, I am rather inclined to suppose him a
> very respectable Man." -Jane Austen, The History of England
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