PCR mutagenesis-strange results

Susanne Rohrer srohrerNOSPAM at immv.unizh.ch
Mon Nov 22 10:08:26 EST 1999


Hi Frederik

The same thing happened to me when I made a pET expression construct. My
primer had a nucleotide insertion by accident (I don't even know where
it came from, since I designed my primers from a file where the sequence
was ok). Two out of three clones did not have the insertion. On the
other hand, I guess if someone wanted to make a directed frameshift
mutation, he would have to sequence several clones to get one that is
right.

Lucky you!





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