Yeast 3-hybrid problem
mayr at SALK.EDU
Mon Nov 22 12:32:42 EST 1999
Yes I streaked untransformed CG1945 and Y190 on -His and -Trp and -Leu
plates: No growth on -Leu or -Trp, on -His w/o 3-AT (as expected) both
strains grow, but when I add 3-AT in the concentration recommended,
> I feel obliged to ask the obvious question - does your host strain
> grow on -His media without transformation with a plasmid?
> (ie. has your yeast stock become contaminated with a HIS strain?)
I did that: I transform both strains with empty (= no insert) bait and
prey vector and they start growing.
> Try a test transformation using a HIS
> plasmid and streak the untransformed and the transformed cells on
> +/- His media.
Just another question: Can high humidity in the incubator have an
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