High-throughput transfection of 293 cells

John McWhirter jmcw at prolifaron.com
Mon Nov 22 11:47:25 EST 1999


I am trying to set up a system for transfecting 293 cells in 96-well
plates. Does anyone know if plasmid isolated with the QIAprep 96 Turbo
miniprep kit is suitable for this application (this would be the same
quality as QIAprep Spin Miniprep Kit plasmid prepared using a vacuum
manifold)? Qiagen tech services says they haven't tried this but have
heard from some customers that it can be done.

Thanks,

John McWhirter
jmcwhirter at prolifaron.com





More information about the Methods mailing list