High competent cells for transformation

michael c lu mlu at sc3103.med.buffalo.edu
Mon Nov 22 14:37:52 EST 1999


Does anyone have a good protocol for making highly competent cells for
transformation.  I'm trying the Hanahan method on a particular strain that
can replicate plasmids with repeats.  However, the FSB buffer doesn't seem
to work for this strain but does a good job with DH5 alpha cells.  I'm
wondering if anyone has used RbCl on other strains besides DH derivatives
and has found success with it.

                                                                            
   Mike




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