primer melting point?

Dr. Duncan Clark Duncan at
Wed Nov 24 04:13:59 EST 1999

In article <81eobl$aut$1 at>, daniboyton at writes
>Can anyone please tell me what the melting points for GC and AT pairs in
>primers are. I believe they are 2 and 4 degrees respectively but I could
>be wrong. 

Other way around. 4 degrees per GC, 2 degrees per AT. Seer later post
for more detail.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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