"Ghost" bands in plasmid preps?
Simon.Dawson at nottingham.ac.uk
Wed Nov 24 12:06:49 EST 1999
Does anyone ever see what I can only call "ghost' bands if they
over-expose an ethidium bromide stained agarose gel of plasmid DNA? They
usually appear to run somewhat faster than what I would think is the
supercoiled DNA band and you only seem to see them if you overexpose the
Any thoughts? Ignore them (they don't seem to cause any problems with
subsequent manipulations)? I'm curious to know if anyone else sees something
Dr. Simon Dawson
School of Biomedical Sciences
Queen's Medical Centre
TEL:+44 (0)115 9709419 FAX:+44 (0)115 9709969
Email:Simon.Dawson at nott.ac.uk
"Back off man, I'm a scientist!" - Bill Murray.
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