"Ghost" bands in plasmid preps?

Simon Dawson Simon.Dawson at nottingham.ac.uk
Wed Nov 24 12:06:49 EST 1999


Hi all,
        Does anyone ever see what I can only call "ghost' bands if they
over-expose an ethidium bromide stained agarose gel of plasmid DNA? They
usually appear to run somewhat faster than what I would think is the
supercoiled DNA band and you only seem to see them if you overexpose the
gel.
    Any thoughts? Ignore them (they don't seem to cause any problems with
subsequent manipulations)? I'm curious to know if anyone else sees something
like it.
    Thanks,


            Simon.

-- 
Dr. Simon Dawson
Floor D
School of Biomedical Sciences
Queen's Medical Centre
Clifton Boulevard
Nottingham
NG7 2UH
U.K.

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