Cloning in pGEM-T

Christophe Jacob jacob at scbiol.u-nancy.fr
Wed Nov 24 12:49:16 EST 1999


Does anyone know if the purification of PCR fragments (from agarose gel or
with a column to eliminate other bands and/or oligonucleotides) before
cloning in T/A vectors is a critical step ??

Thanks for yours answers.
Regards,

Christophe.

-- 
Christophe JACOB
Lab. Forest Biology
University Nancy 1
BP 239
54506 VANDOEUVRE
email: jacob at scbiol.u-nancy.fr




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