Cloning in pGEM-T
Hiranya S. Roychowdhury
hroychow at NMSU.EDU
Wed Nov 24 15:37:04 EST 1999
At 06:49 PM 11/24/99 +0100, Christophe Jacob wrote:
>Does anyone know if the purification of PCR fragments (from agarose gel or
>with a column to eliminate other bands and/or oligonucleotides) before
>cloning in T/A vectors is a critical step ??
>Thanks for yours answers.
>Lab. Forest Biology
>University Nancy 1
>email: jacob at scbiol.u-nancy.fr
Yes, if you want a clean cloning. Furthermore, the unused primers and the
dNTPs may prove inhibitory.
Dr. Hiranya Sankar Roychowdhury
GENE LAB/ EPPWS
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu
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