Gel filtration vs gradient

Tyson tyson at
Thu Nov 25 20:52:56 EST 1999

...perhaps there are disulphide bridges linking the monomers?

"Pierre Rodrigues" <pirod at> wrote in message
news:383D4A53.373A6085 at
| Hello,
| We are trying to look at oligomers of an integral protein. We compared
| gel filtration (by HPLC) to sucrose gradients and obtained opposite
| results: in HPLC, most of the protein was eluted on a 300-400 kDa range
| whereas gradient elution was around monomer size!
| As our protein is an integral membrane protein and has 2/3 of its lenght
| hydrophobic, could there be lipids still interacting with it and make it
| float on gradients?
| We did this experiments on Triton x-100 and CHAPS and did a 100000xg
| clarification before.
| If anyone has some explanations (even partial!) it would be great...
| Thanks
| Pierre Rodrigues
| Institut Pasteur

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