High competent cells for transformation

MIKHAIL F ALEXEYEV INNA at prodigy.net
Thu Nov 25 21:42:01 EST 1999


Check if your strain is recBC (recBC, sbc strains are capable of propagating
palindromes).  RecBC strains do not replicate colE1 well.  Therefore,
apparent low efficiency of transformation may be attributable to something
else.

michael c lu <mlu at sc3103.med.buffalo.edu> wrote in message
news:81c64b$ah7$1 at prometheus.acsu.buffalo.edu...
> Does anyone have a good protocol for making highly competent cells for
> transformation.  I'm trying the Hanahan method on a particular strain that
> can replicate plasmids with repeats.  However, the FSB buffer doesn't seem
> to work for this strain but does a good job with DH5 alpha cells.  I'm
> wondering if anyone has used RbCl on other strains besides DH derivatives
> and has found success with it.
>
>
>    Mike






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