Cloning in pGEM-T

Nam, sangjune pincraft at
Fri Nov 26 07:16:36 EST 1999

i  use pGEM-tT vector (promega) and say to U...don't mind that step

But i recomemded simple :gene clean as agarose-removing

and  ligation on -4 degree overnight....

namely...pGEM-T vector's efficiency is low..but pGEM-T easy vector is
more better

gook luck...

i think my comment is not good...bye
On Wed, 24 Nov 1999 18:49:16 +0100, jacob at
(Christophe Jacob) wrote:

>Does anyone know if the purification of PCR fragments (from agarose gel or
>with a column to eliminate other bands and/or oligonucleotides) before
>cloning in T/A vectors is a critical step ??
>Thanks for yours answers.
>Christophe JACOB
>Lab. Forest Biology
>University Nancy 1
>BP 239
>email: jacob at

More information about the Methods mailing list