Cloning in pGEM-T
pincraft at chollian.net
Fri Nov 26 07:16:36 EST 1999
i use pGEM-tT vector (promega) and say to U...don't mind that step
But i recomemded simple step...eg :gene clean as agarose-removing
and ligation on -4 degree overnight....
namely...pGEM-T vector's efficiency is low..but pGEM-T easy vector is
i think my comment is not good...bye
On Wed, 24 Nov 1999 18:49:16 +0100, jacob at scbiol.u-nancy.fr
(Christophe Jacob) wrote:
>Does anyone know if the purification of PCR fragments (from agarose gel or
>with a column to eliminate other bands and/or oligonucleotides) before
>cloning in T/A vectors is a critical step ??
>Thanks for yours answers.
>Lab. Forest Biology
>University Nancy 1
>email: jacob at scbiol.u-nancy.fr
More information about the Methods