Cloning in pGEM-T

Nam, sangjune pincraft at chollian.net
Fri Nov 26 07:16:36 EST 1999


hi....
i  use pGEM-tT vector (promega) and say to U...don't mind that step

But i recomemded simple step...eg :gene clean as agarose-removing
method.

and  ligation on -4 degree overnight....

namely...pGEM-T vector's efficiency is low..but pGEM-T easy vector is
more better

gook luck...

i think my comment is not good...bye
On Wed, 24 Nov 1999 18:49:16 +0100, jacob at scbiol.u-nancy.fr
(Christophe Jacob) wrote:

>Does anyone know if the purification of PCR fragments (from agarose gel or
>with a column to eliminate other bands and/or oligonucleotides) before
>cloning in T/A vectors is a critical step ??
>
>Thanks for yours answers.
>Regards,
>
>Christophe.
>
>-- 
>Christophe JACOB
>Lab. Forest Biology
>University Nancy 1
>BP 239
>54506 VANDOEUVRE
>email: jacob at scbiol.u-nancy.fr





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