John T Jones
jjones at scri.sari.ac.uk
Fri Nov 26 11:57:35 EST 1999
Andy Scotter wrote:
> We have just cloned a small insert (145bp) into pET42 and
> transformed BL21(DE3) cells with it. They grow fine on plates containing
> LB, 1% Glucose and 15micrograms/ml Kanamycin LBGK), with a reasonable
> transformation efficiency. However, I can't get a liquid culture growing
> using LBGK. I've tried 2.5ml, 5ml and 10ml cultures in Universals (i.e.
> plenty of air) and I'm incubating the culture at 37 degrees centigrade.
> Anyone got any ideas?
You could try growing at 30. This sometimes helps. Dont remember the reasoning
off the top of my head I'm afraid.
More information about the Methods