BL21(DE3) problems

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Sat Nov 27 09:04:30 EST 1999


In article <383FC439.6522D047 at mbio.aau.dk>, T. Max M. Soegaard
<tmms at mbio.aau.dk> writes
>I never saw that before.. interesting!
>Why? What is the effect of the yeast proteins? Is there a reference on this?

I've seen it published somewhere but can't remember where. Yeast extract
reportedly contains IPTG like substances so that you induce expression
ever so slightly. That may be enough for lethality. Of course the extra
glucose should switch the lac of even more but ...

You can supposedly use casamino acids instead of yeast extract.

Duncan

 
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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