BL21(DE3) problems

Dr. Duncan Clark Duncan at
Sun Nov 28 05:49:48 EST 1999

In article <0.58fd35e5.25717a47 at>, DOCTORHIM at AOL.COM writes
>Glucose is irrelevant since  the LacUV5 promoter is used for T7 RNA 
>polymerase.  UV5 is not catabolite repressible.

That's something I wasn't aware of. Useful snippet. Thanks.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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