SOE-PCR

[Esbjorn Fiers]

Hello,
Does anyone have experience with SOE-PCR (splice extension overlap) with
3 fragments? I would like to know a good protocol and get some hints for
primer design (length of overlap, ...).
Thanks in advance,

Esbjorn Fiers

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Esbjorn Fiers
DEPARTMENT OF GENETICS                         Fax:32 (0)9 2645349
UNIVERSITY OF GENT, K. L. Ledeganckstraat 35, B-9000 Gent, Belgium
Vlaams Instituut voor Biotechnologie                           VIB
mailto:esfie at gengenp.rug.ac.be        http://www.plantgenetics.rug.ac.be

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