Piero Morandini wrote: > > 3 Concerning thrombin or factor Xa, has anyone thought of making > a GST or His tag version of the enzyme, so to be able to remove it > (or to purity it easily) after reaction. > (I am of course thinking of using the his-tag version of thrombin > to cut the fusions with GST. Yes, Pharmacia has done it. Check their web page. -Rafa