Cloning problems-Help!!

Dr. Duncan Clark Duncan at nospam.demon.co.uk
Tue Nov 30 05:15:38 EST 1999


In article <3841655C.A7E7D861 at nefo.med.uni-muenchen.de>, zimprich
<zimprich at nefo.med.uni-muenchen.de> writes
>    In the last few months, i have a very big cloning problem,. 
>    I try to get a AOC1 (5´overhang CC!TGAGG), ECO R1 (also 5´overhang 
>    G!AATTC) fragment into a  vector also cut with AOC1, ECO R1.  It 
>    sounds quite easy, but it is a nightmare. 


Aoc I recognition site is really CCTNAGG. Are you sure you have the same
subsequence CCTGAGG both in the insert and vector and not say CCTGAGG in
one and CCTTAGG or whatever in the other? Have you tried any other iso.
of Aoc I i.e. Bsu36 I etc.?

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
DNAmp Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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