SOE-PCR

[stan.forier at agr.kuleuven.ac.be]

Hi,

I've made a few constructs using SOE-PCR and it's very easy to do.
The only thing you need to do is to work out a good PCR strategy to merge
the different fragments.
The reference I used was:
Mutagenesis and Synthesis of Novel Recombinant genes Using PCR.
In: PCR primer, A LAboratory Manual
Ed. by Dieffenbach CW & Drehsler GS (CSHL Press 1995)

Hope you succeed but that shouldn't be a problem. :-)
Stan

Esbjorn Fiers wrote:

> Hello,
> Does anyone have experience with SOE-PCR (splice extension overlap) with
> 3 fragments? I would like to know a good protocol and get some hints for
> primer design (length of overlap, ...).
> Thanks in advance,
>
> Esbjorn Fiers
>
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> Esbjorn Fiers
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