FK506 (tacrolimus) in vitro

Axel Allera allera at uni-bonn.de
Tue Nov 30 11:49:52 EST 1999


Try to dissolve it in DMSO

Axel Allera

Bruce Smith wrote:

> Paula Lavery wrote:
>
> > We are having problems getting FK506 into solution to use in some in vitro
> > assays. It readily dissolves in EtOH (0.2M) but it precipitates out whenever
> > we try to dilute it further into culture medium. We tried adding some Tween
> > 80 to the solution but it didn't help. Anyone have any bright ideas?
> >
> > Paula Lavery
> > Transplant Immunology Lab.
> > Royal Victoria Hospital
> > McGill University
> > Montreal (QC) Canada
> >
> > "If we knew what we were doing it wouldn't be called research, would it?"
> > -- Albert Einstein
>
> My first thought was "how is it normally done?" and "how are you actually doing
> it?"
> I used cyclosporine a long time ago and what we used to do is slowly add diluent
> (culture fluid etc) to the etoh-csa.  Are there other solvent you can use
> (DMSO).  WHat temp is the culture fluid?  does serum play a role either pos or
> neg in the precipitating out?
> markH
> hey i'm alumni at mcgill--great school, great town, (is bob murgita still
> there?)
> Peace
> markH





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