Cutting with MboII

[Martin Chan]

I also encounter the problem when using MboII. Yet, instead of an unexpected
additional faint band above an intense band, a smear is found around the
intense band.
Frank Bartel wrote:

> Does somebody have experiences with the restriction enzyme MboII. We try
> to cut PCR products, but the digestion seems to be incomplete, even when
> incubating overnight.
>
> Thank you for your help.