ATG starts & stops??

[Alan Lee]

Hi netters,

	I have a weird findings from my 5'RACE and I am not quite sure if it is an
artifact or something else. After cloning my 5'RACE product into a vector,
sequencing results showed that the first ATG is followed by a TGA stop
codon! It is not likely to be sequencing error since sequencing of the
genomic clone of the same region gave the same TGA. Any comments and inputs
are welcomed. Thanks a lot.

Regards,
Alan
---------------------------------------------
Alan Lee, PhD.
 
EG Rm.E26
Zentrum für Molekulare Neurobiologie (ZMNH)
UKE University of Hamburg
Martinistr. 85
20251 Hamburg
Germany
 
e-mail: ywlee at zmnh.uni-hamburg.de
Tel: (49) 40-42803-6294
Fax: (49) 40-42803-6302