small fragment library construction

Chris Boyd chrisb at hgu.mrc.ac.uk
Fri Oct 1 04:58:08 EST 1999


Tom Knight (tk at pasteur.ai.mit.edu) wrote:
: chrisb at hgu.mrc.ac.uk (Chris Boyd) writes:
: 
: > Homologous recombination in the host will generate the desired
: > constructs. Because your inserts are so small, the process could be
: > quite efficient. With a suitable design of primers, you could even try
: > IVC and conventional cloning with the same PCR fragment pool.
: 
: Kids, remember to use a RecA+ strain if you do this at home.

RecA is not necessarily required.  Read the references I gave in the
previous post.


Best wishes,
-- 
Chris Boyd                      | from (but not \  MRC Human Genetics Unit
Christopher.Boyd at hgu.mrc.ac.uk  | much longer)  /      Crewe Rd, Edinburgh
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd          EH4 2XU, SCOTLAND



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