small fragment library construction
chrisb at hgu.mrc.ac.uk
Fri Oct 1 04:58:08 EST 1999
Tom Knight (tk at pasteur.ai.mit.edu) wrote:
: chrisb at hgu.mrc.ac.uk (Chris Boyd) writes:
: > Homologous recombination in the host will generate the desired
: > constructs. Because your inserts are so small, the process could be
: > quite efficient. With a suitable design of primers, you could even try
: > IVC and conventional cloning with the same PCR fragment pool.
: Kids, remember to use a RecA+ strain if you do this at home.
RecA is not necessarily required. Read the references I gave in the
Chris Boyd | from (but not \ MRC Human Genetics Unit
Christopher.Boyd at hgu.mrc.ac.uk | much longer) / Crewe Rd, Edinburgh
http://www.hgu.mrc.ac.uk/Users/Christopher.Boyd EH4 2XU, SCOTLAND
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