His TAG protein purification

Raimo J Pollanen rpollane at UTMMG.MED.UTH.TMC.EDU
Mon Oct 4 16:18:19 EST 1999

I have started a project to purify a protein (recombinant ) His Tag from
E coli and after Nickel (Novagen) column I have still big proteins
(double band around 100-150kD) which follows even after  ion exchange
and gel filtration steps...? How could I get eid of that ? My own
protein is sized aroun 21-22kD??


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