Small RNA electrophoresis: any tips?

rogier stugerNOstSPAM at cellbiology.uni-frankfurt.de.invalid
Tue Oct 5 11:23:03 EST 1999


Hiya,
just take 6% acrylamide, 7M urea.
I used this to resolve RNAs in the 60 to 500 nt range.
mind that ethidium bromide doesn't stain RNA very effectively (cos it's
singlestranded), so you need about 0.5 micrograms in a band to see it.
Good luck,
Rogier Stuger
rogier at biogate.com


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