Strange problems with Invitrogen TOPO vectors

Earl Blewett blewett at
Tue Oct 5 16:39:05 EST 1999

Greg Hawkins wrote:

> Has anyone had problems with TOPO vectors (especially the TA cloning
> vectors)?  We sequence these clones for customers and have found restriction
> sites that flank the cloning site missing, sequencing primers that do not
> work on one side of the cloning site, plus other strange things we can not
> explain.  Is the topoisomerase causing so strange recombination that screws
> up the regions flanking the cloning site?

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