jrt at home.com
Tue Oct 5 19:15:32 EST 1999
Floating out of the well is indeed usually a symptom of residual EtOH.
But probably unrelated to your RNAse problem. Consider that RNAse A
alone is generally not sufficient to reduce your RNA completely to
NTs. Some short oligos will remain. Try adding a dash of RNAse T1 to
the mix. I agree the possibility that it might be DNA is plausible
Merck & Co.
Rob Kirkpatrick <kirkpat at cc.umanitoba.ca> wrote:
>Does anyone know the common cause(s) of inhibition of RNAse in DNA
>preps? The DNA was prepared from Concert columns and repeatedly fails
>to digest with RNAse. The RNAse batch works on other samples but not
>this one. I also seem to have some problem with loading this DNA on a
>gel to check it - it wants to float up and out of the wells. I don't
>know if the problems are related or not. I've considered the presence
>of residual ethanol as a problem but haven't come to a conclusion on
>that yet. The DNA sample is about 1.5 mg/ml and the RNA band is easily
>visible but quite faint in comparison to the DNA. I think that is about
>all the info I have. Any help would be appreciated.
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