Isopropanol vs EtOH

V.S. Gautam gautam at mcbl.iisc.ernet.in
Wed Oct 6 10:54:16 EST 1999


Please read up current protocols in molbio. OR maniatis. Its given in
thes.
-gautam
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On Wed, 6 Oct 1999, Rob Kirkpatrick wrote:

> Howdy,
> 
> Does anyone know why isopropanol is used to precipitate DNA in some
> cases (i.e. after column purification) while Ethanol is used other
> times?  I'm told it has do do with yield (EtOH is better?) and purity
> (isopropanol is better?).  Also, while we're at it, why do we sometimes
> use Sodium Acetate and other times use Ammonium Acetate to aid in
> precipitation?
> 
> Thanks in advance!
> 
> Rob
> 

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